Confocal microscope – material reflective mode Single Molecule sensitivity – Multiphoton PMT/APD Confocal volume – manual or controlled by dia of fiber FCS & FILM LED – ns- 265,280,295,300,320,335,340,345,370,460, 485,520nm Lasers – ps 370,405,440,473,488,514,635,660,780,830nm Galvo scanning / piezo sample scanning Nikon/Zeiss/Olympus
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Stimulated Emission Depletion Ring Laser & excitation laser Phasar plot/FILM Sample Piazo scanning Confocal volume motorised Z focus motorised Nikon Microscope Choice of excitation lasers Choice of objectives.
Single Molecule sensitivity – PMT/APD Multi photon ps/ns lasers/LED LED – ns- 265,280,295,300,320,335,340,345,370,460, 485,520nm Lasers – ps 370,405,440,473,488,514,635,660,780,830nm Confocal volume motorised FCS & FILM z focus motorised Image scanning XY Galvo or Piezo stage scanning Objectives 10x,20x,50x,100x, oil & water Microscope – Inverted Nikon/Zeiss/Olympus
Single Molecule sensitivity – Multiphoton PMT/APD Confocal volume – manual or controlled by dia of fiber FCS & FILM LED – ns- 265,280,295,300,320,335,340,345,370,460, 485,520nm Lasers – ps 370,405,440,473,488,514,635,660,780,830nm Galvo scanning / piezo sample scanning Nikon/Zeiss/Olympus Objectives 10x,20x,50x,100x, oil & water